IL-5 anticorps

N° du produit ABIN5689047

Cet anticorps Lapin Polyclonal détecte spécifiquement IL-5 dans WB, ELISA et Neut. Il présente une réactivité envers Humain.

Aperçu rapide pour IL-5 anticorps (ABIN5689047)

Antigène: IL-5 (IL5) (Interleukin 5 (IL5))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-5 est non-conjugé

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Détail du produit anti-IL-5 anticorps

Purification: Anti-hIL-5 specific antibody was purified by affinity chromatography employing immobilized hIL-5 matrix.

Immunogène: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-5 (human Interleukin-5).

Information d'application

Indications d'application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-5 (0.45 ng/mL), a concentration of 0.05 -0.09 μ,g/mL of this antibody is required.

ELISA:

To detect hIL-5 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-5.

Sandwich:

To detect hIL-5 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-5 (XP-5191Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-5.

Western Blot:

To detect hIL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: -20 °C

Stockage commentaire: IL-5 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Détails sur IL-5

Antigène: IL-5 (IL5) (Interleukin 5 (IL5))

Autre désignation: IL-5

Sujet: Interleukin 5 (IL-5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL-2, IL-4 and growth hormone. Human and mouse IL-5 have 70 % amino acid sequence homology.

ID gène: 3567

UniProt: P05113

Pathways: Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour